UDC 628.355.2:631.461.2
DOI 10.35776/VST.2024.10.05
Wang Weize, Makarova S. V.
Temperature effect on nitrification processes in activated sludge
Summary
Temperature has been an important factor affecting the efficiency of biological nitrogen removal by activated sludge at wastewater treatment facilities. A laboratory SBR reactor was used in the experiments; activated sludge for cultivation was obtained from aeration tanks of the municipal wastewater treatment facilities. The taxonomic composition of bacteria in activated sludge was determined by high throughput sequencing of the DNA section encoding 16S rRNA. The nitrification rate was determined at water temperatures of 1, 5, 8, 11, 17, 19 and 21°C. Nnitrifying bacteria Nitrosomonas spp. and Nitrospira spp. were found in activated sludge with the abundance of 0.07% and 0.34%, respectively. The maximum nitrification rate observed at 21 °C was 4.3 mg N–NO3 per 1 g of ash-free substance of activated sludge per hour. The nitrification rate decreased with decreasing temperature and was 0.3 mg/(g·h) by N–NO3 at 1°C. The results of the study can be used as a theoretical basis for optimizing the operation of the treatment facilities in areas with low temperatures to improve the efficiency of nitrogen removal from wastewater.
Key words
wastewater , nitrification , activated sludge , temperature , biological removal of nitrogen