Methodic aspects of microbiological water analysis

Summary

Ensuring the epidemic safety of the water use in relation to intestinal waterborne infections has been the main and high-priority task of the microbiological water quality control. The regulations in force have methodological differences in determining and identifying epidemiologically significant parameters the validity of which is of primary importance. Taking into account the urgency of the issue the methodological discrepancies of oxidase test procedure in determining total coliforms on Endo agar are considered. On the ground of experimental data a new procedure of differentiating coliforms from oxidase positive microorganisms like Pseudomonas, Aeromonas and other aquatic saprophytic bacteria is suggested. Chromogenic media are of great practical interest since they contain substances that inhibit the growth of gram-positive microbial flora that is abundant in natural water sources and produces a negative effect in identification of target microorganisms. In this respect possible use of Chromocult Coliform Agar ES chromogenic medium in sanitary-microbiological water analysis for total and thermotolerant coliforms and Escherichia coli is evaluated. An original color differentiation of the colonies growing on Chromocult Coliform Agar ES medium is suggested that requires additional tests only for separate colony types. The comparative analysis of Endo and Chromocult Coliform Agar ES media is given.

Key words

Escherichia coli , microbiological water analysis , oxidase test , Endo medium , chromogenic medium , Chromocult Coliform Agar ES , coliforms

“Water Supply and Sanitary Technique” Magazine, CONTENTS №9 (part 2) 2007

PROBLEMS, PERSPECTIVES
E. I. Pupyrev. The Water Code of the Russian Federation and Development of Technical Regulation System An attempt is made to analyze systemically the Water Code and the Law On Technical Regulation and to assess their impact on water management quality in Russia.	2
V. V. Petrashkevich. Testing of Conic Two-Chamber Fish Protection Facility The results of hydraulic-biologic tests demonstrate high reliability of the conic two-chamber fish protection facility.	9
A. S. Kopylov. Modern Methods of Complex Design of Waterside Ground Water Intakes The modern methods of complex design of waterside ground water intakes are considered.	13
E. Yu. Borovitskaya. Analysis of Ground Water Monitoring Results The results of monitoring during the whole period of water intakes operation, as applied to situations associated with sharp lowering of ground water levels and reduction in water wells output prior to taking a decision about new wells drilling are analyzed.	17
V. G. Chvyrev, A. F. Karniz, M. V. Semenov. Potable Water Disinfection under Extreme Situations The experience in potable water disinfection with the help of small electrolysis units to provide temporary water supply under extreme situations is presented.	25
S. M. Galkin Treatment Facilities of the WAVIN-Labko OY Co. (Finland) The experience of the Finnish firm WAVIN-Labko OY, a leading manufacturer of wastewater treatment systems, in cooperation with Russian enterprises is presented.	29
I. A. Ivleva, L. S. Alekseev, A. V. Boldin. Barrier Functions of Ground Water Treatment Technologies for Domestic Purposes Possibilities of the use of modern water treatment methods as the best accessible technologies for treatment of ground water buried at rather small depth (up to 120-140 m) in the systems of domestic water supply are analyzed.	33
A. N. Belevtsev, S. A. Baykova, V. I. Zhavoronkova, N. N. Melnikova, V. I. Nosenko. Evaluation of Efficiency of the Use of Ground Brucite Aquamag for Water Treatment Technologies The results of laboratory tests (conducted at the NII VODGEO Co.) of chemical and physical properties of two fractions of ground brucite Aquamag used as a reagent for removal of different chemical substances from natural and waste water are presented.	41
L. Styazhkova. Perm Sewerage System: 90 Years The history of creation and development of the sewerage system of the city of Perm is presented.	44
Abstracts of the Articles Published in the Issue № 9, part 2	48

Methodic aspects of microbiological water analysis

Summary

Journal VST